Confocal microscopy after indirect immunofluorescent staining with monoclonal anti-TM antibody and secondary FITC-labeled antibody.

Cells transfected with plasmid pRSVSVOTMwt, show a high concentration of the TM-anti-TM complexes in the plasma membrane (A, B). In contrast, cells transfected with plasmid pRSVSVOTMmut show a much reduced membrane incorporation of the TM antigen and a high juxtanuclear staining (C-E). Panel E represents an enlarged photograph of a cell expressing the mutant insT 1689.