Erythroid and megakaryocytic progenitors transduced with the MFM vector develop into colonies in the presence of AP1903 alone.

Immediately after transduction with the MFM vector, CD34+ cord blood cells were plated in colony assays with different combinations of drug and cytokines. Displayed in panels A-C are representative BFU-E colonies with the following conditions: (A) BFU-E assays with AP1903 alone; (B) BFU-E assays with AP1903, SCF, and IL-3; (C) BFU-E assays with epo, SCF, and IL-3; and (D) a representative megakaryocytic colony from plasma clot assays plated in AP1903 alone. The cells were stained for CD41 expression, as detailed previously.