The MFM vector can mediate a dramatic expansion of mouse bone marrow cells in the presence of AP1903.

(A) MFM is an MSCV-based retroviral vector. The gene encoding the F36Vmpl fusion protein is transcribed from the long terminal repeat (LTR). The neo gene is transcribed from the phosphoglyceate kinase (PGK) promoter. In Figure 1A, M indicates myristylation domain; HA, epitope tag from influenza hemagglutinin; F36V, the CID-binding domain; and mpl, the intracellular signaling domain of murine mpl. (B) Mouse bone marrow was transduced with the MFM vector and then incubated in the absence (○) or presence (•) of AP1903 (100 nmol/L). The transduced mouse bone marrow cells cultured in the absence of AP1903 died over a 2-week period, while the cells cultured in the presence of AP1903 exhibited a dramatic expansion, similar to our previously published results using FK1012.10