Fig. 3.
Immunostaining of platelets with MARCKS and CD41a antibodies.
Intact platelets were fixed, permeabilized, and immunostained with either human MARCKS mouse monoclonal antibody (C and D) or human CD41a (fibrinogen receptor) mouse monoclonal antibody (E and F). The second antibody used in both cases was affinity-purifed Cy3-conjugated donkey Fab2 fragment raised against mouse IgG (dilution 1:200). Panels A and B show control platelets incubated only with the second antibody.
