American Society of Hematology

Fig. 4.

$Fig. 4. Binding characteristics of the soluble GM-CSF receptor identified in PBSC and bone marrow products. / Plasma conditioned by PBSC products was subjected to Con-A sepharose and ligand affinity column chromatography and eluted fractions were pooled, dialyzed against distilled H2O lyophilized, and resuspended in 500 μL PBS. 150 μL of each of these samples was pooled and volume reduced in a centrifugal filtration cartridge (Ultrafree® Biomax-5K NMWL membranes, Millipore Corp, Bedford, MA).125I-GM-CSF saturation binding experiments were performed on these pooled samples. Shown are Scatchard analysis and saturation binding curves (inset) of a representative experiment (n = 3).$

Binding characteristics of the soluble GM-CSF receptor identified in PBSC and bone marrow products.

Plasma conditioned by PBSC products was subjected to Con-A sepharose and ligand affinity column chromatography and eluted fractions were pooled, dialyzed against distilled H₂O lyophilized, and resuspended in 500 μL PBS. 150 μL of each of these samples was pooled and volume reduced in a centrifugal filtration cartridge (Ultrafree® Biomax-5K NMWL membranes, Millipore Corp, Bedford, MA).¹²⁵I-GM-CSF saturation binding experiments were performed on these pooled samples. Shown are Scatchard analysis and saturation binding curves (inset) of a representative experiment (n = 3).

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