p0p mAbs are directed against mouse GPIb-IX.

(A) Immunoprecipitation from surface-biotinylated resting platelets by p0p 1-5. NP-40 lysates were incubated with nonimmune rat IgG1 (control) or p0p 1-5, followed by protein G-Sepharose. Proteins were separated by 9% to 15% gradient SDS-PAGE under reducing conditions, transferred to a PVDF membrane, and detected by streptavidin-HRP and ECL. (B) Unlabeled platelet proteins were immunoprecipitated with nonimmune rat IgG1 (control) or p0p 1-5, followed by SDS-PAGE and immunoblotting with FITC-labeled p0p 5. Bound p0p 5 was detected by HRP-labeled rabbit anti-FITC.