Fig. 6.
Activation of allogeneic T cells by B-chronic lymphocytic leukemia (CLL) cells in the mixed lymphocyte reaction.
B-CLL cells were cultured for 48 hours in medium alone, with DSP30 at 2 μmol/L, with CD40LF, or with DSP30 and CD40LF. Cells were washed twice, irradiated, and co-cultured with freshly separated allogeneic T cells. After 5 days, culture at a stimulator to responder ratio of 1:2 cells were pulsed with 1 μCi 3H-thymidine. The proliferative index (3H-thymidine incorporation/3H-thymidine incorporation of T cells alone) was calculated. Data represent mean proliferative index ± SEM of 4 independent experiments.
