Transplantation of SL-ICs into secondary recipients.
Lanes 1-7: CD34++CD38− leukemic cells from patient No. 6 were injected at the indicated doses into NOD/SCID mice. After 6-8 weeks, these mice were sacrificed, and the percentage of engraftment was estimated by Southern blot analysis. Lane 8: Pool from the bone marrow of 8 mice injected with 2 × 104 CD34 cells from patient No. 2. Lane 9: Human CD45+ cells present in primary engrafted mice were sorted; 2 × 105 cells were injected into secondary recipients; and after 6 weeks, engraftment was estimated by Southern blot analysis. Lane 10: Pool from the bone marrow of 8 mice injected with 10 × 106 unsorted cells from patient No. 4. Lane 11: Secondary recipient mouse injected with 2 × 106 CD45+ cells from the previous pool of mice. Lane12: Pool from the bone marrow of 9 mice injected with 2 × 105 CD34+ cells from patient No. 5. Lanes 13 and 14: Secondary recipient mice injected with 2 × 105 and 2 × 106 CD45 cells, respectively, from the pool of mice shown in lane 12.
