Cytotoxic activity and phenotype of IL-2–activatedW/W NK cells.

(A) Spleen cells were depleted of red cells, B cells, and T cells, and the remaining cells were used to generate A-LAK cells. Day 7-10 A-LAK cells were used as effectors against YAC-1 cells (○) or EL-4 cells in the presence (▴) or absence of α-CD90 antibodies (•). All assays were done in duplicate or triplicate. (B) Day 7-10 A-LAK cells were stained with antibodies for CD3^FITC, NK1.1^PE, B220^TRIC, and the expression of B220 on NK1.1⁺ CD3⁻NK cells was calculated. Data are representative of 3 independent experiments. Similar data were obtained with IL-15 (n = 2; data not shown).