Cytotoxic activity of purified W/W NK cells.

Spleen cells were depleted of red cells, B cells, and T cells and stained with IL-2Rβ and DX5. Double-positive NK cells were sorted and plated at the indicated E/T ratios with 2500-5000 YAC-1 target cells. All assays were done in duplicate or triplicate. Results are representative of 2 independent experiments. The spontaneous release was less than 10% of the maximal release.