NK cells in W/W chimeras.

BM (A) and spleen cells (B) were red-cell depleted and stained with a combination of antibodies for IL-2Rβ^FITC, NK1.1^PE, TCRαβ^biot, and CD19^biot. Absolute cell numbers (×10⁶) are indicated on top of each dot plot and refer to the mean ± SD of 7 chimeras per group. An electronic gate was set to exclude TCRαβ⁺ T cells and CD19⁺ B cells, and the percentage of IL-2Rβ⁺ NK1.1⁺ NK cells was calculated. (C) Spleen cells were stained for DX5^FITC, TCRαβ^biot, and c-kit^PE. The percentages of c-kit⁺ DX5⁺ TCRαβ⁻ NK cells are indicated. Results are representative of 5 independent experiments.