Fig. 2.
Thermostability of NH2-terminal PEGylated variants.
SakSTAR variants were heated for the indicated times and temperatures, and 2 μg protein were separated in a 15% SDS-PAGE gel under nonreducing conditions: (1) SakSTAR, (2) SakSTAR (S3C-SP5), (3) SakSTAR (2SC-SP5, S3C-SP5), (4) SakSTAR (S3C-MP5), (5) SakSTAR (S3C-MP10), and (6) SakSTAR (S3C-MP20).
