Inhibitor neutralization by isolated scFv.

CLB-CAg A and CLB-CAg 117 were diluted to a concentration that corresponded to approximately 2 BU/mL. Increasing concentrations of scFv EL-14 were added, and the mixture was incubated for 2 hours at 37°C. Residual FVIII activity was determined relative to a control sample that was incubated in the absence of mAb. CLB-CAg A (□); CLB-CAg 117 (•).