Fig. 2.
Expression of RhAG glycoprotein in a transcription and translation-coupled reticulocyte lysate system.
Autoradiogram of [35S]-methionine-labeled proteins separated by 12% SDS-PAGE (for details, see Materials and Methods). Lane 1, pLNCXRhAG vector; lane 2, pLNCXRhAG vector with microsomal membranes. Arrow indicates nonglycosylated RhAG; arrow head, glycosylated RhAG.