The engraftment levels of NOD/SCID recipients injected with uncultured or ex vivo–transduced cells.

The percentages of human cells within the marrow of NOD/SCID mice transplanted with uncultured Lin⁻ CB or ex vivo–transduced Lin⁻ CB are shown. Values are derived from flow cytometric analysis where engraftment exceeded 5% and Southern blot quantification of all others. The dose of injected cells is shown on the X axis. Closed circles represent mice containing EGFP⁺ human cells, crossed circles indicate mice in which the human cells did not contain EGFP, and open circles show mice that could not be assessed for human cell transduction.