CpG methylation in the erythropoietin promoter and 5′-UTR.

DNA from normoxic Hep3B, HeLa cells, and peripheral blood mononuclear cells was treated with sodium bisulfite. Polymerase chain reaction amplification was performed, and the DNA sequence was obtained from the complementary strands (guanines converted to adenines). (A) Coding strand sequence from −288 to −133. (B) Noncoding strand sequence from −66 to −289. (C) Coding strand sequence from −288 to −133 from peripheral blood mononuclear cells (PMC1 and PMC2). (bent arrows) Transcription start site. (open circles) Partially methylated CpGs. (filled circles) Fully methylated CpGs. (diamonds) Hemimethylated site present in normoxic Hep3B cells.