American Society of Hematology

Fig. 2.

$Fig. 2. Southern blot analysis of methylation in Hep3B and HeLa cells. / Genomic DNA from Hep3B and HeLa cells was digested with Xba1 and Eag1, fractionated by size, transferred to a nylon membrane, and hybridized to the full-length Xba1 fragment of the erythropoietin gene. The Xba1 fragment is 1250 bp long, and the combined Xba1/Eag1 fragments measure 461 and 789 bp. Nox, normoxia; Hyp, hypoxia for 8 hours.$

Southern blot analysis of methylation in Hep3B and HeLa cells.

Genomic DNA from Hep3B and HeLa cells was digested with Xba1 and Eag1, fractionated by size, transferred to a nylon membrane, and hybridized to the full-length Xba1 fragment of the erythropoietin gene. The Xba1 fragment is 1250 bp long, and the combined Xba1/Eag1 fragments measure 461 and 789 bp. Nox, normoxia; Hyp, hypoxia for 8 hours.

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