Human protein S induces Akt phosphorylation in Tyro3 cells.

NIH3T3-Tyro3 cells (lanes 1-6) and NIH3T3 cells (lanes 7-12) were treated with the vehicle (lanes 1 and 7) and human protein S (lanes 2-6 and 8-12) for various times. Cell lysates were blotted for phospho-Akt (A). Lanes 6 and 12 consist of protein S–treated (30 minutes) cells cultured in the presence of wortmannin. Lane 13 represents serum-starved NIH3T3-Tyro3 cells stimulated with the serum for 30 minutes. The same blot was stripped and reprobed with the antibody recognizing all forms of Akt (B).