EGF induces Akt phosphorylation in EGFR/Tyro3 cells.

NIH3T3-EGFR/Tyro3 (lanes 7-12) and NIH3T3 cells (lanes 1-6) were treated with vehicle (lanes 1 and 7) or EGF (lanes 2-6 and 8-12) for various times. (A) Equal amounts of cell lysates were blotted for the presence of phosphorylated Akt (p-Akt) using a phosphospecific anti-Akt antibody. Lanes 6 and 12 were EGF-treated (30 minutes) cells cultured in the presence of wortmannin. Lane 13 represents the serum-starved NIH3T3-EGFR/Tyro3 cells fed again the serum for 30 minutes. (B) The same blot was stripped and reprobed with an antibody recognizing all forms of Akt. EGF, epithelial growth factor.