The p85 subunit of PI3 kinase interacts with phosphorylated EGFR/Tyro3.

NIH3T3 cells transfected with EGFR/Tyro3 (lanes 6-10) and the parental cells (lanes 1-5) were treated with EGF for various times. Equal amounts of cell lysates were immunoprecipitated with the anti-Tyro3 antibody. Immunoprecipitates were then blotted for (A) Tyro3, (B) PY-Tyro3, or (C) P85. (D) IgG portion of the gel was shown as a loading control. Lanes 11 and 12 were nontreated lysates from NIH3T3 and NIH3T3-EGFR/Tyro3, respectively. EGF, epithelial growth factor.