Western blots of Thr-145 and Met-145 forms of recombinant glycoprotein (GP)Ib under reducing and nonreducing conditions.

(A) Coomassie blue stain of recombinant GPIbα under nonreducing conditions (Thr-145, lane 1, and Met-145, lane 3) or reducing conditions (Thr-145, lane 2, and Met-145, lane 4). (B) Western blot of duplicate of panel A with anti-human platelet antigen (HPA)-2a (lanes 1 and 2), and anti-HPA-2b (lanes 3 and 4).