Fig. 5.
![Fig. 5. Effect of Ado receptor antagonists on fMLP-induced PLD activity. / 1-O-[3H]alkyl-2-lysophosphatidylcholine-labeled neutrophils were preincubated at 37°C for 5 minutes. The cell suspensions were then pretreated with 10 μmol/L CB and increasing concentrations of either CGS15943 (A) or CSC (B) and incubated in the presence or absence of 0.1 U/mL ADA for 5 minutes. Neutrophils were stimulated with 0.1 μmol/L fMLP in the presence of 1% ethanol for 10 minutes. The amount of radioactivity incorporated into [3H]PEt was assessed as described in “Materials and Methods” and is expressed as the percentage of the radioactivity in the total lipid extracts. The data are the means ± SEM of 3 separate experiments.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/95/2/10.1182_blood.v95.2.519/6/bloo00203005ax.jpeg?Expires=1723198750&Signature=VkGj5XiCv2TfsD7eb9rpkSZz7KC0w7IEm0iS8wAf06YcIjzsIv8Vck~KLAxFWEMGuQZ03biOot4kscodsbQTK6Z-GWHCRjsOTxiwwUC5crgldBM1HMuRyqujDtZCWx5258aTcBjRCbnLHcdDwx8zZFSlwgYPQXGr2zCB3xwuuzKiB51WSmj4-ZovVoaz7fUVHKyuAc5yosHO4EBzEej74g9dxyUPigcRavKToNQVkTA9AWd-HlxLkCnXur96TJgRY3Dq0XHmPkjUIWL15ZZjVlRveWE7SBoml6yyLARYtml-An~vUi4utBDCV~cenaHRESvAvVKaaNWL0mglhzfolQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Effect of Ado receptor antagonists on fMLP-induced PLD activity.
1-O-[3H]alkyl-2-lysophosphatidylcholine-labeled neutrophils were preincubated at 37°C for 5 minutes. The cell suspensions were then pretreated with 10 μmol/L CB and increasing concentrations of either CGS15943 (A) or CSC (B) and incubated in the presence or absence of 0.1 U/mL ADA for 5 minutes. Neutrophils were stimulated with 0.1 μmol/L fMLP in the presence of 1% ethanol for 10 minutes. The amount of radioactivity incorporated into [3H]PEt was assessed as described in “Materials and Methods” and is expressed as the percentage of the radioactivity in the total lipid extracts. The data are the means ± SEM of 3 separate experiments.
