Flow cytometric analysis of the infiltrating adult T-cell leukemia (ATL) cells in lymph nodes.

CCR7/EBI1 expression levels of infiltrating ATL cells prepared from lymph nodes of 2 representative patients, patients 26 and 27, and control CD4⁺CD45RO⁺ T cells from reactive lymph nodes of HTLV-1-seronegative individuals were compared by flow cytometric analysis using an anti-CCR7 mAb, CCR7.6B3. The infiltrating ATL cells and control CD4⁺CD45RO⁺ T cells were purified by the negative immunoselection technique as described in Materials and Methods.