Regulation by IL-12 of IPP-induced chemokine expression as determined by ELISA.

Vδ2-enriched cultures were established as described in Figure 6 and cultured with IL-12 and TGFβ, either alone or in combination, in control cultures maintained in IL-2 (IPP × 1) or in cultures that had been stimulated again with IPP (IPP × 2). Supernatants were harvested at 48 hours, and MIP-1α and MIP-1β expression was determined by ELISA. Data shown represent the mean ± SD of 3 healthy donors. The values for IPP × 2, IPP × 2 + IL-12, and IPP × 2 + IL-12 + TGFβ were significantly different from each, with a P > .01.