Chemokine expression as determined by ELISA for Vδ2⁺ T cell lines.

Vδ2⁺ T cell lines were established by FACS sorting at 10 cells per well and expanded in vitro using PHA and IL-2. Cells were then activated by plate-bound anti-CD3 or anti-γδ TCR and anti-CD28 for 24 hours. Control cells were maintained in IL-2 alone. Supernatants were harvested, and chemokine expression was determined by ELISA. Data for 8 different Vδ2⁺ T cell lines are shown. All of the lines produced MIP-1α and RANTES when stimulated with anti-CD3 + anti-CD28. However, MCP-1 was not detected in the same supernatants (data not shown).