Chemokine and cytokine expression in freshly sorted Vδ2⁺ cells in response to IPP and PHA.

Vδ2⁺ cells were FACS sorted from the total PBMCs, seeded at 40 000 cells per well, activated for 24 hours with IPP (30 μg/mL) or PHA (0.5 μg/mL) in the presence or absence of autologous irradiated APC (10 000 cells/well), and supernatants collected. Autologous irradiated APC were also cultured alone or with either IPP or PHA. The levels of MIP-1β and IFNγ in the supernatants were determined by ELISA. Data shown are from 1 representative donor of 2 tested.