Chemokine expression as determined by ELISA in PBMC cultured in vitro in IL-2 or IL-2+IPP.

PBMCs were cultured in medium IL-2 (open bars), IL-2+IPP (hatched bars), or LPS (closed bars) either once (day 1) or 3 times (day 14). Supernatants were harvested at the times shown. The presence of MIP-1α, MIP-1β, RANTES, and MCP-1 in these supernatants was determined by ELISA. Data shown are for cultures that were challenged either once (day 1) or that had been stimulated with these agents at weekly intervals (day 14).