American Society of Hematology

Fig. 4.

$Fig. 4. At least 2 serum components are involved in rosette formation, as demonstrated using anion exchange chromatography. / SDS-PAGE profile of fractions eluted from a Sepharose Q anion exchange column. Aliquots of the unbound fraction (A1) and gradient fractions (GF) before (A) and after (B) pooling and concentration (aliquots of up to 10 μg protein, corresponding to 10 times more gradient fraction than the WS and A1 fractions), were resolved by SDS-PAGE (10% acrylamide) as described in the legend to Figure 2. (C) Effect of the fractions on rosette formation. The rosetting assay was conducted as described in the legend to Figure 1, and the fractions were used at 15% (vol/vol) of A1 and 10% (vol/vol) of GF1-4 for all experiments. Two different batches of serum were fractionated, and the assay was conducted in duplicate for each batch (mean ± SD for 4 experiments).$

At least 2 serum components are involved in rosette formation, as demonstrated using anion exchange chromatography.

SDS-PAGE profile of fractions eluted from a Sepharose Q anion exchange column. Aliquots of the unbound fraction (A1) and gradient fractions (GF) before (A) and after (B) pooling and concentration (aliquots of up to 10 μg protein, corresponding to 10 times more gradient fraction than the WS and A1 fractions), were resolved by SDS-PAGE (10% acrylamide) as described in the legend to Figure 2. (C) Effect of the fractions on rosette formation. The rosetting assay was conducted as described in the legend to Figure 1, and the fractions were used at 15% (vol/vol) of A1 and 10% (vol/vol) of GF1-4 for all experiments. Two different batches of serum were fractionated, and the assay was conducted in duplicate for each batch (mean ± SD for 4 experiments).

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