Phenotypes of human monocytes and of human dendritic cells and macrophages generated in vitro.

The expression of cell surface proteins characteristic for dendritic cells (CD1a, MHC class II, CD83, CD115) and macrophages (CD14, CD36, CD64, CD68), and dendritic cell maturation (CD86, CD25) were investigated by (A) fluorescence-activated cell sorter analysis of monocytes, (B) immature dendritic cells, (C) mature dendritic cells, (D) macrophages (on day 7), and (E) monocyte-conditioned medium–treated macrophages. Cells were generated in vitro and florescence-activated cell sorter analyses performed as described in Materials and Methods. Dot plots: Forward scatter on the x-axis and side scatter on the y-axis. Histograms: Fluorescence intensity in a logarithmic scale (x-axis) was blotted against cell numbers (y-axis).