Injected CD34⁺/CD38⁻ cells are viable and retain their ability to proliferate after single cell transfer.

Cells were attached to RN and injected with OG-dextran. Two hours after injection, cells were detached with peptide and fluorescent cells transferred as single cells to individual wells of 96-well plates. In total, 46 suspension and 75 injected cells were analyzed after 2 injection sessions. (A, C) Percentage of total wells transferred that displayed cells surviving (closed bar) and proliferating (open bar) after transfer. (B, D) Representative sampling of the survival and proliferation of 12 individually transferred suspension (B) or injected (D) cells. Each symbol represents the progeny generated by an individually transferred cell.