Retroviral integration and hβc expression on the FDB1 and FDB2 cell lines.

(A) Schematic representation of an integrated RufNeo-hβc provirus showing the position of BamHI and KpnI restriction sites. Long terminal repeat sequences (LTR) and the neomycin resistance gene under control of the MC1 promoter (MC1Neo) are indicated. (B) Southern analysis of BamHI- and KpnI-digested genomic DNA (as indicated) derived from the FDB1 and FDB2 cell lines, and from clones of each of these lines, with the use of a Neo probe. The sizes of molecular weight markers (EcoRI-digested SPP1 phage DNA) are shown in kilobase. (Note that the band marked with the asterisk is in fact a doublet.) (C) Surface expression of hβc on FDB cell lines. FDB1 and FDB2 cells were stained with an anti-hβc monoclonal antibody 4F3 (solid lines) or an irrelevant isotype control antibody (dashed lines).