The differential rate of migration of FANCA, FANCG, and FANCC proteins.

FANCA antiserum immunoprecipitates FANCA and co-precipitates FANCC and FANCG proteins. Whole cell extracts (WCEs) were prepared from lymphoblast lines including: JY (normal), PD4 (Fanconi group C mutant cells expressing no detectable FANCC protein) and PD4/FANCC (isogenic PD4 cells complemented with normal FANCC cDNA). Extracts (100 μg) were electrophoresed either without prior manipulation (lanes 1, 3, and 5) or following immunoprecipitation (IP) (3 mg for each sample) using affinity purified anti-FANCA anti-serum (lanes 2, 4, and 6). Fanconi anemia proteins in each lane were detected on immunoblots with affinity purified anti-FANCA, anti-FANCC, and anti-FANCG serum as indicated.