Multiplex-PCR genotype analysis of the -globin gene cluster.

(A) Schematic representation of the α-globin gene cluster. Figure indicates extents of each deletion represented in the multiplex-PCR assay and relative positions of the primers (except for the control LIS1-F and LIS1-R primers, which are located on a different chromosome). Locations of sequence homology (X, Y, Z boxes) and hypervariable regions (HVR) are also shown. (B) Representative results from DNA samples with various α-globin genotypes. The M indicates the 1 kb-Plus molecular weight marker (Life Technologies), and the AL, the allelic ladder comprising all 8 possible amplification fragments.