Fig. 5.
![Fig. 5. Binding of LE2E9 to native or Arg2150His C1 and C1C2 domains. / Native and Arg2150His recombinant fVIII C1 domain (A) or C1C2 domains (B) labeled with [35S]methionine and expressed in reticulocyte lysates were incubated for 2 hours at 4°C with LE2E9 (LE) bound to Protein A Sepharose. After washing, bound material was eluted by SDS-buffer and analyzed by SDS-PAGE, followed by autoradiography. Controls included the human monoclonal antibody, BO2C11 (BO), and normal donor polyclonal IgG antibodies (Wi). The experiments were repeated 3 times with similar results.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/95/1/10.1182_blood.v95.1.156/5/bloo00150005w.jpeg?Expires=1725169739&Signature=PIQ1Mo6UFR~v86ItZdJziG11l1z6ruUaJmQLicMUZEcELN-ueLn8Wk3uFG17rR5j2-DjbcRpsr4hUsqIGftOr2gD8cSe747b-1~dEgdVHj20fw~6CyxD3p6uQ~BoFIE6AcA7GbxT8OpXjrWMQqQY~CHaXDTysuYjd9N5VU0CEdWGeulOfvGo7JLHGGVPQKJISkYlSli8gG2-5H8ICksGrqL3kRgt7nmvqPzltxAbP9~vhFsA1FTXP-f3ppyjSP53JbFyFu7xtv4UiUxZ21DWsjPvF7XP9gH2QYXNN6YHWrik4UKrGthzqNlKUkTWiVE7WGqhTVdp1cDsIcJxNU-IOA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Binding of LE2E9 to native or Arg2150His C1 and C1C2 domains.
Native and Arg2150His recombinant fVIII C1 domain (A) or C1C2 domains (B) labeled with [35S]methionine and expressed in reticulocyte lysates were incubated for 2 hours at 4°C with LE2E9 (LE) bound to Protein A Sepharose. After washing, bound material was eluted by SDS-buffer and analyzed by SDS-PAGE, followed by autoradiography. Controls included the human monoclonal antibody, BO2C11 (BO), and normal donor polyclonal IgG antibodies (Wi). The experiments were repeated 3 times with similar results.
