Fig. 3.
![Fig. 3. Immunoprecipitation of wild-type and mutated rfVIII light chain. / Recombinant fVIII fragments labeled with [35S]methionine and expressed in reticulocyte lysates were incubated for 2 hours at 4°C with LE2E9 (LE) bound to Protein A Sepharose. After washing, bound material was eluted by SDS-buffer and analyzed by SDS-PAGE, followed by autoradiography. Controls included a human monoclonal antibody, BO2C11 (BO), directed toward the fVIII C2 domain,19 a rabbit polyclonal IgG antibody (aA3), directed toward amino acid residues 1797-1815 of the fVIII A3 domain and normal donor polyclonal IgG antibodies (Wi). The experiments were repeated 3 times with similar results.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/95/1/10.1182_blood.v95.1.156/5/bloo00150003w.jpeg?Expires=1725169063&Signature=G3~Bi1AGZSzIx1Q5qddbNGmd76mn5qH2eW87B3CuuZHzOCezY2qAZeAj3OBobhbnBrpUWLoHHC8ZwQxQ3zrBnFcKYBodoMKaaLsWA7LP2KbHN2Ad2nBwMt9LBmS2k2N9SLdBOU8nrzywQhSlyBg0kwh-5Rnya6Izx6eE-cxIHz6ZsjKyt7m9k7R0gQwAEt7cJbS8ej5XU54oL7~7qQuYR-qcEmGPJ7wD9WQ6QDeUNvHBoPm8dwuon3~uN6iFdqrWKNhcM1Q9WEJBQyWmAaTeLSlz-j87Sl4Q1ZDUEnUdqqVsoioj71V009aL2GvCr7mbvCCOHyu0PWJtxhU5pV-Dqg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Immunoprecipitation of wild-type and mutated rfVIII light chain.
Recombinant fVIII fragments labeled with [35S]methionine and expressed in reticulocyte lysates were incubated for 2 hours at 4°C with LE2E9 (LE) bound to Protein A Sepharose. After washing, bound material was eluted by SDS-buffer and analyzed by SDS-PAGE, followed by autoradiography. Controls included a human monoclonal antibody, BO2C11 (BO), directed toward the fVIII C2 domain,19 a rabbit polyclonal IgG antibody (aA3), directed toward amino acid residues 1797-1815 of the fVIII A3 domain and normal donor polyclonal IgG antibodies (Wi). The experiments were repeated 3 times with similar results.
