Fig. 2.
Procaspase-3 and PARP, but not IκBβ, processed during PDT-induced apoptosis.
HL-60 cells were treated with verteporfin (100 ng/mL) and red light (2 J/cm2). Cell lysates were prepared at increasing times and subjected to sodium dodecyl sulfate–polyacrylamide gel electrophoresis and Western immunoblot analysis. Bands were detected using a chemiluminescence detection system and visualized by autoradiography. Lanes designated 0 minute contain lysates of samples prepared immediately after light irradiation.
