Selective reduction of DC number after anti-CD3ɛ administration. BALB/c mice were treated on day 0 with control antibody or anti-CD3ɛ (50 μg IV) and analyzed on days 2 and 15 for I-E^d, CD45R, and CD11c expression. Pooled low-density spleen cells from 3 individuals in each group were analyzed by 2-color immunofluorescence after staining with PE-labeled anti-CD45R and FITC-labeled anti–I-E^d (A through C) or PE-labeled anti-CD45R and FITC-labeled anti-CD11c (D through F).