Capacity to generate DC precursors from NK1.1⁺ cells.

13 dpc FL-derived HPCs were co-cultured with PA6 cells + SCF + Flt3L in transwell plates. At the indicated time points, the nonadherent cells were collected and NK1.1⁺ cells were sorted and recultured with PA6 cells + GM-CSF + SCF + Flt3L. After being recultured for 10 to 12 days, the nonadherent cells were restimulated with GM-CSF + mTNFα for an additional 3 to 5 days. The expression of Ia and CD86 antigens on these cells was examined as described in “Materials and Methods.” Solid and dotted lines indicated the immunofluoresence intensity of cells stained with a control and the test MoAbs, respectively. The quads were set up on the isotype-matched control dot plot. Representative results from 3 independent experiments are shown.