Immunophenotypic analysis on NK1.1⁺ cells generated from FL-derived Lin⁻c-kit⁺ HPCs.

Lin⁻c-kit⁺ HPCs from 13 dpc FL were cultured in the presence of PA6 cells + SCF + Flt3L for 14 days. The phenotypes of the nonadherent cells were analyzed by 2- and tri-color immunofluorescence staining as described in “Materials and Methods.” (A) In the tri-color analyses, expression of asialo-GM1 and CD3ε or CD4 antigens on NK1.1⁺ cells was analyzed. (B) The 2-color analysis was performed on expression of NK1.1 and Ly49c antigens from the cultured cells. The quads were set up on the isotype-matched control dot plot and the results are representative of 3 independent experiments.