Generation of NK1.1⁺ cells from FL-derived Lin⁻c-kit⁺ HPCs.

13 dpc FL-derived Lin⁻c-kit⁺ HPCs were cocultured with PA6 cells in the presence or absence of SCF + Flt3L with or without GM-CSF in a transwell culture system. The nonadherent cells were collected at the indicated time points and stained with FITC-conjugated anti-NK1.1 MoAb. The data represent mean value ± SD of NK1.1⁺ cell percentage in the cultures from 5 independent experiments. Black squares represent PA6 cells; red diamonds, PA6 cells with SCF and Flt3L; and green circles, PA6 cells with GM-CSF, SCF, and Flt3L.