Immunofluorescence analysis on DC precursors generated from murine FL-derived Lin⁻c-kit⁺HPCs.

Immunofluorescence analysis was performed on the nonadherent cells from FL-derived HPCs cocultured with PA6 cells + GM-CSF + SCF + Flt3L for 12 to 14 days. The indicated markers of FITC-labeled MoAbs were used to demonstrate the phenotypic characteristics of the DC precursors. Solid and dotted lines indicated the immunofluoresence intensity of cells as a control and the test of MoAbs, respectively. Representative results from 3 independent experiments are shown.