American Society of Hematology

Fig. 5.

$Fig. 5. The capacity of the cultured cells to enhance allogeneic MLR. / Allogeneic MLR was performed using purified T cells (3 × 105 cells/well in 96-round-well plate) as responder cells. The unfractionated nonadherent cells, which were generated from FL-derived HPCs cocultured with PA6 cells + GM-CSF + SCF + Flt3L for 12 to 14 days before and after restimulation by GM-CSF + mTNFα for an additional 3 to 5 days, were treated by MMC and used as stimulator cells at the indicated cell numbers. DCs derived from BM-derived HPCs stimulated with SCF + Flt3L + GM-CSF + mTNFα, and peritoneal macrophages were used as controls. The proliferation of T cells was measured using MTT after 5 days of culture. Results are expressed as the mean ± 1 SD of triplicate cultures and are representative of 3 independent experiments. Black squares indicate macrophages; red diamonds, DCs from BM HPCs; green circles, DCs from FL HPCs; and purple triangles, FL-derived DC precursors.$

The capacity of the cultured cells to enhance allogeneic MLR.

Allogeneic MLR was performed using purified T cells (3 × 10⁵ cells/well in 96-round-well plate) as responder cells. The unfractionated nonadherent cells, which were generated from FL-derived HPCs cocultured with PA6 cells + GM-CSF + SCF + Flt3L for 12 to 14 days before and after restimulation by GM-CSF + mTNFα for an additional 3 to 5 days, were treated by MMC and used as stimulator cells at the indicated cell numbers. DCs derived from BM-derived HPCs stimulated with SCF + Flt3L + GM-CSF + mTNFα, and peritoneal macrophages were used as controls. The proliferation of T cells was measured using MTT after 5 days of culture. Results are expressed as the mean ± 1 SD of triplicate cultures and are representative of 3 independent experiments. Black squares indicate macrophages; red diamonds, DCs from BM HPCs; green circles, DCs from FL HPCs; and purple triangles, FL-derived DC precursors.

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