The culture of murine 13 dpc FL-derived Lin⁻c-kit⁺ HPCs.

(A) Using a phase contrast microscope, morphologic analyses were performed on cultured murine 13 dpc FL-derived Lin⁻c-kit⁺ HPCs stimulated with GM-CSF + SCF + Flt3L + mTNFα at day 7, 14, and 21. Original magnifications: × 200. (B) 2-color immunofluorescence analysis was performed on the cultured cells at the indicated time points. The cells were sequentially stained with biotinylated CD86 MoAb and PE-labeled Ia MoAb. CD86 was revealed by FITC-streptavidin. The quads were set up on the isotype-matched control dot plot. These results are representative of 3 independent experiments.