Nucleotide sequence of the fibrinogen γ-chain gene exon VI. The PCR-amplified γ-chain genes of the normal control (A) and the propositus (B) were directly sequenced by dideoxy termination method using the reverse primer. The nucleotide position 2900, indicated by the arrow in (B), of the propositus' gene was heterozygous for A and G. This nucleotide substitution changed the γ153Cys→Arg as shown in (C). The underlined nucleotide is located in the intron region.