Fig. 6.
RT-PCR reaction.
One representative RT-PCR reaction of 4 shows a reduction in the levels of mRNA for flt3/STK mRNA in Ik7-infected CD34+ cells compared to control-infected CD34+ cells. The RNA was obtained after flow cytometry sorting of the cells as in Figure 4. The PCR product was blotted and hybridized with a 32P-labeled cDNA probe. In this experiment, the same amount of cDNA was amplified with primers specific for IL-2Rγ and results show no significant alteration of mRNA levels by Ik7.
