Analysis of Ik7-infected CD34⁺ cells.

(A) Representative flow cytometric analysis of CD34⁺ cells cultured for a total of 4 days and having been infected with virus for 2 days (pre-sort). Reanalysis of CD34⁺EGFP⁻ and EGFP⁺ cells from these cultures (CD34⁺ E⁻ and CD34⁺E⁺), which are being used to assay DC hematopoiesis. (B) RT-PCR analysis of Ikaros mRNA in the sorted CD34⁺ cell populations showing the presence of the multiple endogenous Ikaros isoforms Ik1 (888 bp), Ik2/3 (630 bp), Ik4 (500 bp), and the 467 bp-specific product corresponding to Ik7. (C) Detection of DC activity by MLR. Stimulator cells were obtained after Ik7 infection and culture in FKGm17 of CD34⁺ EGFP⁺ cells (closed squares) or CD34⁺ EGFP⁻ cells (open diamonds) and the response of purified T cells was measured as CPM after³H-thymidine incorporation.