Reversion of the amifostine antiapoptotic effect in CD34⁺ cells by the κB decoy oligonucleotide. CD34⁺ cells (purity, >96%, ≤98%; 10⁵/mL) were incubated in 10% FCS-RPMI, with or without a cytokine mix (see Materials and Methods), amifostine (8 μg/mL), decoy, or scrambled oligo (5 μmol/L) for 16 hours. Apoptosis was then analyzed by propidium iodide incorporation in cell DNA. The results represent the means ± SE of 4 separate experiments.