Impairment of the amifostine antiapoptotic effect in 32D cells by the κB decoy oligonucleotide. 32D cells (2 × 10⁵/mL) were incubated without WEHI-3 CM and with or without amifostine (100 μg/mL), 7.5 μmol/L κB decoy, or scrambled oligodeoxynucleotide for 18 hours. Cell apoptosis was then analyzed by propidium iodide incorporation in cell DNA. The results represent the means ± SE of 4 separate experiments. In cells incubated with WEHI-3 CM, either with or without amifostine and/or oligonucleotides, apoptosis was less than 4%.