Fig. 3.
Effect of VBL on chemotaxis of HUVEC. Cells (1.2 × 105) exposed for 24 hours to each VBL dose were seeded in the upper compartment of Boyden chambers, whereas the CM of NIH 3T3 cells was placed as the chemoattractant in the lower compartment. Nonexposed cells were used in the positive and negative controls. The latter were without the chemoattractant. Cells that had migrated to the lower surface of the filter separating the compartments after 6 hours were counted after coding the samples. Bars represent the mean ± 1 SD of the number of migrated cells in 5 to 8 400× fields of the filter per sample in 1 representative experiment out of 5. Significance by the Wilcoxon rank test.
