Tc1 and Tc2 clones differentially regulate Th1/Th2 development.

Tc1 (■) and Tc2 (○) clones were cocultured with autologous PBMCs previously depleted of CD8 cells (CD4 + APC) at varying ratios and in the presence of SEB at 2.5 μg/mL. After 6 days, cultures were stimulated overnight with PMA/ionomycin in the presence of Brefeldin A and stained with CD3 APC, CD8 PerCP, IL-4 PE, and IFN-γ FITC. By gating on CD3⁺CD8⁻cells, cytokine production of CD4 effector cells could be examined. Results are expressed as percentage of CD4 cells positive for IL-4 or IFN-γ.